Proteome Response of Meretrix Bivalves Hepatopancreas Exposed to Paralytic Shellfish Toxins Producing Dinoflagellate Gymnodinium catenatum

Paralytic shellfish toxins (PSTs) contamination of seafood has become a growing global problem. However, the molecular response bivalves, some most popular seafoods, to PSP seldom been reported and underlying mechanisms interactions between Meretrix meretrix bivalves PSTs-producing dinoflagellates are scarcely known. This study compared protein expression profiles toxin-contaminated non-PSP toxin contaminated M. meretrix, determined proteome responses identified potential biomarkers based on feeding experiments. Results showed that content total in was 40.63 ± 4.08 μg saxitoxin (STX) equivalents per gram, with 95.3% hepatopancreas, followed by gill (1.82%) foot (1.79%). According two-dimensional gel electrophoresis (2-DE), 15 differentially expressed proteins (at least 2-fold difference) hepatopancreas without were detected. Eight them successfully MALDI-TOF MS. These catalase, ultraspiracle homolog, G2 S phase-expression protein, paramyosin, Mn-superoxide dismutase, regulator receiver domain-containing sarcoplasmic calcium-binding major facilitator superfamily transporters. The differences levels last three involving cell signaling, structure membrane transport 4.2, 5.3 4.9-fold, respectively. could be further developed as biomarkers. other two up-regulated proteins, dismutase involved defence against oxidative stress, suggesting acts xenobiotics poses stress bivalves. gives insights into toxin-producing dinoflagellate at proteomic level using 2-DE develop specific markers